Star sprinters Yes Yes Yes and Nature Strip will face off down the straight in the Group 1 Black Caviar Lightning at Flemington.
Leading New South Wales trainer Chris Waller confirmed the decision on Thursday despite previously saying it was unlikely they would face off.
It sets up a tantalising battle over 1000m down the Flemington straight on February 15.
James McDonald will ride Nature Strip while champion hoop Glen Boss has retained the ride on Yes Yes Yes.
"I have no doubt he (Nature Strip) is going to prove very tough opposition for Yes Yes Yes in the Lightning and both horses will certainly be tested by the great depth we have in the Australian sprint ranks at the moment," Waller said.
Both will also be set for a European campaign, with Waller reiterating his desire to take Nature Strip and Yes Yes Yes to Royal Ascot.
Waller is keeping his options open with Yes Yes Yes' preparation, but said The Everest winner's final run will be in the G1 T.J. Smith Stakes at Randwick.
Waller said he is happy with both speedsters, who had a trial together at Warwick Farm last Tuesday over 900m.
Yes Yes Yes will have another piece of solid work in the next few days before heading to Melbourne ahead of a February 7 jumpout.
"Fortunately we are able to make use of the Flemington straight for this jumpout and although he has won on the straight course earlier in his career, I feel it is important to further his experience in straight racing as we head towards the G1 Lightning Stakes for his first-up run where Glen Boss will ride again," Waller said.
Waller said Nature Strip had returned in 'excellent order'.
"(He) remains very relaxed and continues to mature each preparation which is very positive," Waller said.